In vivo assays
Middle Cerebral Artery Occlusion (MCAO)- stroke model
One of our major indications where we use our newly developed molecules is the recovery after ischemic stroke. As a widely accepted stroke animal model we perform MCAO on young adult rats and follow their stroke scores (focal and general deficits) for several weeks. We either directly inject our inhibitors to the damaged brain tissue or, more recently, we introduce our inhibitors into the arterial blood flow with catheters close to the occlusion site. We also test the cognitive and emotional functions of the rats after several weeks when the motoric functions of both the treated and the control animals are recovered. During the first three weeks we determine the physiological status of the damaged brain areas using MRI, SPECT and several immunohistochemistry staining techniques.
Rat heart and respiratory functions
We follow the effect of the new inhibitors on general physiological functions (heart rate, blood flow, oxygen saturation, breath rate) of young adult rats using MouseOx collar sensor. We inject the inhibitors intraperitoneally and continuously detect the vital signs for one hour. We also use MouseOx to monitor our subjects when our inhibitors are directly dropped on the surface of the brain of a mouse with mosaic expression of GFP in its neurons.
We assess the sensory and motor functions of the animals relative to their general well-being based on a multi-dimensional scoring system supplemented by video recordings, image analysis, and advanced statistics.